FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_20_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_20_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166113
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC200.007331290351.779821
TAGTAGA500.00771718127.5992774
CACCAGG500.00771718127.5992776
AAGTAGT850.00409191620.305811
TGAGCAG2152.2404687E-514.4414825
GAGCAGG1450.005190970414.2754886
GTTATCA1951.5775945E-414.1620011
AGAGAGG3651.0510121E-813.23651469
CTCGCTT2204.401701E-412.54512622
ACCAGAG5257.2759576E-1212.48914669
GACCAGA6950.012.41348469
GAGGATC2100.004425128511.50316169
AGAGGAT2752.2386119E-411.29401368
TCTGATA2752.2448844E-411.2906138
AGACCAG7950.010.85203968
CCAGAGA5151.13330316E-710.05130767
CAGAGAG4906.222763E-79.85985268
GAGACCA8950.09.63952167
ACGAGAC10150.08.83720765
AAGCCTA4805.21798E-58.62477429
Produced by FastQC (version 0.11.2)