FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_19_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_19_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126111
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACTG252.55913E-455.1968235
CCGATAC200.007345601551.74702519
TCCCGTA200.007345601551.74702538
GTGACCC200.007345601551.74702517
CCAATAG350.001343643139.4263043
AATAGAT400.002583312334.51172
AACCAGT400.002588345234.4980166
ATCCGGG501.8526873E-434.49801630
ACGAGCC501.8526873E-434.49801652
GTACAAT450.004599618330.6770691
CTCTAAT450.004599618330.6770691
ATAGATC450.00460855730.6649063
CAATGCT605.397033E-428.7483464
CAATAGA500.00769650327.609361
CCACGTG500.00771142427.59841213
AAGACCG658.617125E-426.5369385
TATTCAA700.001324653324.6512172
CTCGGAG851.4339373E-424.3515423
AATGCTG1004.3070666E-420.6988095
CATCCGG900.00570864219.16556529
Produced by FastQC (version 0.11.2)