FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_18_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_18_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119680
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGT200.00734301851.7528
GGCTGTA200.00734301851.7568
TCTAGAC350.001342988339.428573
GCGTCAG350.001342988339.428574
AGCCAAT400.002587088234.554
ATGTGTA450.004606328430.66666611
GTATAAG450.004606328430.66666615
GCAGCGT450.004606328430.6666661
TATAAGA450.004606328430.66666616
AGCGTCA500.0077077127.5999983
GTCAGTT500.0077077127.59999835
AGATGTG700.001326879624.6428579
GGCAGGA750.001982665623.037
GATGTGT950.007794569218.1578966
CCAGAGA2503.5895755E-715.1868
CAGAGAG2454.8673337E-614.08163369
ACCAGAG3002.8032136E-612.6569
AGAGAGG2050.003693348511.78048769
AGACCAG3602.1321564E-510.54166767
GACCAGA3652.4828803E-510.39726166
Produced by FastQC (version 0.11.2)