FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_16_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_16_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448304
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA700.001332992724.6415445
CTGTACT1202.4534202E-622.9987744
ATAGAGT750.001991755622.9987744
TTACCCT1253.4969562E-622.0788234
GTGTTAG800.002886364721.5757881
GTATAAG1004.3306834E-420.69889615
CTATGCA850.00411493920.2952989
TTAGGGT850.00411759820.2930364
TAGACGT850.00411759820.2930365
ACTATGC1056.012775E-419.7154338
TACAGTG900.005730842219.1656447
CTCTAAC900.005730842219.1656442
GTATTAT1451.2553022E-519.0462131
TGTAGTA950.00782959918.1569272
TAGGAGA1351.5748691E-417.8879344
TACCATC1200.001475403717.249087
TAGGCAT1200.001475403717.2490847
TTGGGGG1200.001475403717.249084
CCTCTAA1250.001930156416.5702061
GGCGTTT1250.00193881616.55911619
Produced by FastQC (version 0.11.2)