FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_15_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_15_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227480
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGTA252.5634823E-455.197012
GTATACG200.00734667251.7585751
CCTCTTA500.00772413827.59850525
GCGTCAG658.6384016E-426.5370244
CATAGGC809.532324E-525.87359851
GTGTATA1151.68525E-624.0039771
ATAGGCA902.1198532E-422.99875552
GCATAGG750.00198876622.99875550
GGCATAG953.0562925E-421.78829249
CCAGACC800.002893274921.561333
TATGCCG1304.8861984E-621.23428557
ATGTGTA850.004111480420.29301811
ATAAGTG1056.0048327E-419.7132175
AGGCATA1108.212007E-418.81716348
GTATAAG950.00780818418.1609021
TAGAACT950.00781809318.156914
CTTGGCT950.00781809318.156914
GTATGCC1402.0707138E-417.2528656
CGTATGC1452.7069604E-416.65793255
CTCAAAC1300.002514287415.92221453
Produced by FastQC (version 0.11.2)