FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_14_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_14_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92926
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG150.002344723669.0296260
GTGACAC252.5562628E-455.1939733
TGAGGCT200.007341045451.7443474
CCCAAGT400.002585505634.4962320
CTCTCTA2253.3923483E-513.80592369
CTCTACA1500.00645110713.80592369
CCTCTCT2701.8680416E-411.50493669
GACCTCT3752.7299284E-611.04473969
ACCTCTC3207.466167E-510.78587768
AGGCTTA2700.00242756710.2211066
TTAGGCT2750.002826792210.03526810
CGAGACC5907.383278E-99.94494569
GGCTTAG2850.0037993639.6831537
AGACCTC4301.3975281E-59.6320468
ACGAGAC6302.1578671E-89.313519568
CTTAGGC3050.00664400039.0481929
GCTTAGG3100.00759266748.9022538
GAGACCT5101.0385951E-48.12113267
CACGAGA7453.2327807E-77.875862667
CCACGAG7806.719929E-77.52245866