FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_13_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_13_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134240
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGG150.00235140868.9970230
CTACGGA200.00734620951.74776538
TTACTCT400.002588754334.498514
TCAAGAC553.2426606E-431.3622843
TCGTTAC450.004609280330.66534212
CATGACG450.004609280330.66534214
CCAATAA450.004609280330.66534213
TCGGCAG450.004609280330.6653425
GCTTTAC450.004609280330.6653421
AGGAGGC658.619311E-426.5373157
TACTCTT658.619311E-426.5373155
CTACATC1359.236828E-925.56397669
TCTACAT1651.7982711E-618.82438368
TCTCTAC2654.060348E-815.62778969
CTCTACA2001.12352245E-515.53011767
CTCTCTA3102.7665374E-713.35923968
AGACCTC6200.012.80260469
GAGACCT6850.011.58775868
ACCTCTC4251.2309178E-59.74438766
CCTCTCT4251.2309178E-59.74438767
Produced by FastQC (version 0.11.2)