FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_12_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_12_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210516
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTTA306.301472E-445.99730752
GTATAAG602.8968134E-740.24764315
GTATTAC350.001342559239.4450041
TGTGTAT702.1756932E-839.42626612
GCAGCGT709.654577E-734.5143781
GTGTATA953.1844684E-729.05093213
GGTCTGG605.394182E-428.7619821
TGTATAA854.4076187E-628.410114
ACTAGGT500.00771406327.6049423
TAACATG500.00771406327.6049424
CGGCCGT500.00772301527.59838313
AGGGGAG500.00772301527.59838310
GCGTCAG1155.58357E-827.0048354
AGCGTCA906.861539E-626.8381393
TATAAGA906.8742247E-626.83176416
CAGCGTC1052.2662054E-523.0041162
ATGTGTA1052.2703745E-522.99865211
AGATGTG1304.8928414E-621.2295279
GTGTTGT1154.5650446E-521.008751
GAGACAG1451.2574354E-519.0333721
Produced by FastQC (version 0.11.2)