FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_11_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_11_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147641
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGGG200.007347113551.7487348
GAACCCC200.007347113551.7487341
CTTACGA200.007347113551.74873414
GTCCGCA450.004610337330.66591554
AACAGTG450.004610337330.6659157
AAATGCG450.004610337330.6659157
GTTGTAT500.00771437927.5993231
GAACGGT500.00771437927.59932311
CAAATGC750.001985134322.9994356
AGAACAG900.005712077519.1661975
ACAATCC900.005712077519.1661978
CTTCATG1200.001468782717.24957529
GCTTCAT1503.4998317E-416.09960628
AGAACTG1350.003225720715.3329575
AGAGACA1605.7170674E-415.0933817
GGAGAAA1450.00518728314.2755111
GGCTTCA1709.051175E-414.20553427
TAGGCTT3205.7948746E-611.8590845
TATTCCC3105.481101E-511.12875825
GGGAATA2802.6494035E-411.08901320
Produced by FastQC (version 0.11.2)