FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_10_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_10_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250238
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT200.0073548751.74550229
TATTGTA605.412989E-428.7475012
GTCTTTA500.007704236527.6141571
ACAGACG500.00772682427.597618
TAGCACA658.6424797E-426.5361564
TTAGGCA750.001989698322.99800140
TAAGCCA902.1211476E-422.99800134
GAATATA953.0457717E-421.8006521
TAGCAGC850.00411339220.29235519
AACTGCG850.00411339220.2923557
TACAGTG850.00411339220.2923555
GCTCCAT900.00572502419.1656
TATTGGA950.00782169818.1563172
GCACAGT950.00782169818.1563176
TTCTAAT1200.00147326917.24852
GCAGCGC1200.00147326917.248521
TTGGGTT1250.001936024216.5585614
CTGCCCC1756.329543E-515.770069
CATGGGG1605.730843E-415.09545512
TGGGGCA1605.730843E-415.09545514
Produced by FastQC (version 0.11.2)