FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2594_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2594_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length75
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGAGGTCACTAGGGGTCACCAAAGGTCACGAGGGGTCACCAGCGGCGCGCTCGGCTCCCCAGCGCCCAGCCCG11.1904761904761905No Hit
CCCATAGAGTCCCCCAATGCTTTTTGAGCCATCTCTGGATAGGGATTCCGACAGTGTTCCGTATCTGTCTCTTAT11.1904761904761905No Hit
CTGACACCCTCTCTTTGTCTTTAAGCTCTTCCAGGTACTGAAGAGGCCCACTCGAAGTACTGAGGCTGCCCCGCT11.1904761904761905No Hit
TTCTAATTCCGATTTCGAAAAATATGGTACAAGGATTTCGAAGTTTCAAAAATATTTGATTTCAAGAGAAGAGGT11.1904761904761905No Hit
CGATCACGTCTTCTGGGGTAGGACTTCTGTCTTTGCGAACCTTTTCTTCCTGTCTCTTATACACATCTCCGAGCC11.1904761904761905No Hit
ATCGTTGACACGTTCAACTTACCGTGAAGCAAGAAGAAGCCTGCATCTTCAATCATTAAGCCGTATAGTAGAAGC11.1904761904761905No Hit
AAGTGAAACCGATAGACATGGAATTGGCAGTCGAATGTCAAATGGAGACGGAATCCGATGAGAATTCCCTACCAA11.1904761904761905No Hit
ACACCGGAGAATCGCTTGAGATCCAATATCAGCGGCAGCCAAAGATTTTTCTGGGAATTTTCTATTTAGAACGAC11.1904761904761905No Hit
GTTAGGGGGTTATTAAAACTTTTTTTGAATTATTTTTTATTTGAACTTTATCGCGGGAGGTCTCCGTGAAAATTT11.1904761904761905No Hit
GGTGACCCCATGTGACCTCTGGTGACCCCTAGTGACCTCTGGTGCCCCCATCGGACCTCAGGTGACCCTGTCACT11.1904761904761905No Hit
CCCCAATACATGATATTCTATATGGCTACAATGCTCATATCTTTTTTCGTCACATGTTTCGGAATCCGGGTTTCC11.1904761904761905No Hit
ATACATCAATGTTTGGTTTGACGTTGATTGCAATTGGTGAGATTTCTTCTCCGGCGAAACCGACGTTGAGCTTAA11.1904761904761905No Hit
CCTAAAAAGGAAAAAAGAAATGGACCCAGCTAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGAG11.1904761904761905No Hit
CAAAAAAACACCAAATGCTCACTGAATAATACAATCATAATAAAACAAAATCTTACACATAAACAAATCTCAAAT11.1904761904761905No Hit
AGAATATAGTTTCCATTGTTATCTGTTGGAAGTGGAGATCCATCTTGAGACAATGGTTTACCACTTGCATCAGTT11.1904761904761905No Hit
ATTGATCACTTTTGCCAAAAATGGGAGCGTAGTTTTTTGAAAAATCAAGCAAAGGTCCAAAATATCAAAATGAAG11.1904761904761905No Hit
ATCCTTTAGTGATTGCTCCATTGTCCTCCACAACCACGTTACAACCGATCTTATGTTTGTTTGAGTCGTCACCCC11.1904761904761905No Hit
TTGCTAGGTTCATTGAAAACGCCACCAATCTAAGAAAAAAAAAGAAAATTCAAAAAGAAACCTAAATAAACTCAC11.1904761904761905No Hit
ACTTAATCACATCGCGGCGAATACAGTCATCACGCGTTAGCGCAATACCACGCCCTGTCTCTTATACACATCTCC11.1904761904761905No Hit
CCCTTGGATGACAGAACCTTCATTGATTTTGTACCATTTCTTGAGACTCTCTAGCTATTAGAATATTTTTTTCAG11.1904761904761905No Hit
CCTGTTACACTAGACTCCGCCCCTTTTCGTCTCCAAATGTGCTGCGTGATGCTTTTTTCGACACGTTACGTCAGC11.1904761904761905No Hit
TAAATGCATTCATGATCCATTTCTACTATATCCTTATTTCAAAACTTCTCCATTTCCACGTCATCACGACCTAAT11.1904761904761905No Hit
GGAGGGGGGCGTGGGCTTTGAAACGAGTGTGGGGCTGCTGGTCCCCCGGAGTTGCATTCTGATCTGAAAAGAAGA11.1904761904761905No Hit
GTCAGAGCCGAAGTCCTGAGGTCGGTTATTTAGAATGAAGACATCATTTCACCCAAAGATCCGAAAGTGTTTAAT11.1904761904761905No Hit
AGATTGAGGGATAAGTAGACAGTTTTCAACTTCTCTTCCTCGATGGGAAGAGATGCAACACGTGGCAAGATGAAT11.1904761904761905No Hit
AGTGTATACTGAAAAATGGATTTTTCTTTGGAATCGCTTTCCGAATTAAAAGTTTACCCTCGAATAAACGGGCAC11.1904761904761905No Hit
GTATGGGTGAGATTCAAAAAAGACTATTTATAAAACTCTTCGATTACAAAAAGGCAGTCATAAATCTAAGAGAGA11.1904761904761905No Hit
ATCTAATGTAAACTCATCTGATAACCGTGTCTCTCATCGCAATAGCTGTCTCTTATACACATCTCCGAGCCCACG11.1904761904761905No Hit
CCGTTGGTGGTGGATACTTTTCCGGAAGGATCAGGTGTCGGAATGAGATCGGGAGGACAGTCTCCGCTCCTATCC11.1904761904761905No Hit
GGTTAGTTTCAACGTTCTCTATAAAAAGTCAACCAGTCAATCTTAGTCTTGAGATGACCTTCTCTCTCTCTCTCT11.1904761904761905No Hit
CACGATGACAGCTCAAGGAGGGTACAGTCAACTGGCGAATGGATATCCGTCTCCAGTGGTTCAGGACCCCGGGAC11.1904761904761905No Hit
GTGGAAATAAACTCAAAAATCTGTAGTTTTTTGAATTTTAAATGGTTTCGTTTCAGATGAAAAAACTGTGAAATA11.1904761904761905No Hit
GTAATAATAAAGTCATCATTATCATTACACTTTGAGTGTATATACTAAGGGATCAAGGGGATTGAGCTGTCTCTT11.1904761904761905No Hit
CCAAAGGTCACTAGGGGTCACCAGAGGTCACAGGGGGAACTAGGGGTAACCCAGGGTCAACAAAGGGAAAGAGCG11.1904761904761905No Hit
TTAATTAATTCAGAACAATTCCAAAAAAAGACATTCGTAAAATGAAACACAAACAGAATACAAATAAATATCGAG11.1904761904761905No Hit
ATCTACGCGACAACAAGTGGGAAGACGCAGCTAGGAAAAGTCGAATCTCTCTACTTTCACGAGAAATTCGCGGAA11.1904761904761905No Hit
ACTATTAACTGCATTCTGGCATCGTTCGATCCGAATTTACAATTGAAAGCTACTTGTGAATTGATGTGTGATCCA11.1904761904761905No Hit
GAATAGCGATAAATTGTGGGAAAAGGTGTGTGGAGGAACGAATAGGTAAACATGAAGATGGAATATTTACTGATA11.1904761904761905No Hit
ATTTAAAATAAAGTGTACTATCGATTTTTCAATACAATTCATATTGTTGTCTCCCCTAGAGGAGACGTTCCAAAA11.1904761904761905No Hit
ACCCATTTGTTGGGCGACTACTTGGCTTTGCCTCTCTAATTGAGAAGAAAATGCTTTCTTTTCCAGAATAGTACC11.1904761904761905No Hit
CTCTCAACGTGGAGCCAATTTTTAGTTCAAATATCAGTTGAACTGTTGGTTCTTTCGACTGTCTATATTCTGTCT11.1904761904761905No Hit
TCACTAGTGAGAGTCAGATCAGTTTCATTCTGTGAGTCGTCGATGGCAGTTGTAGACTCAGTTCCAATGCTGACG11.1904761904761905No Hit
GATGAGGAGATCAGGGATGAGCCGATACGAAGGGAAGAAGCGTAGAGAAGAAGGAAACGGTAGTAAAAGGAAGAG11.1904761904761905No Hit
ACCAATGATTAAGACAGTGGTTCCCAACCTTAAAAAACACCTTCTCCAAAAGTGTGATCTCACATGACATTTCGG11.1904761904761905No Hit
CAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTC11.1904761904761905No Hit
TTTTTGTGTCGATTAGCTCTTCTTTTTTGTGAGCGAAGCCTCCCCAAAGCAGGAAAACAATCGGTTTTTCGGATT11.1904761904761905No Hit
TCTATACACCGTCTAGGGAGAATGTAGAAGGCGTTTTGAGAGATGTAGCTAGACTGAAGATCTCAATTGGAGACA11.1904761904761905No Hit
GGATCATCATCGTACTGGTTTTGGTAATTGTGTTGCTTGTGATTCAAAAGTGATAAAATTAGTGTGACAAACCTG11.1904761904761905No Hit
CCCATGGAGATCCAATTCGAAATCATGTTCGTAATGGTTCTATATTCCTGTCTAGCAGTCACCGGAGTCGGCGGA11.1904761904761905No Hit
ACACTATCTTTGGAAGCTAGACTACTATGTGAACTTTCAGAAACAGAGAGTTAGATGATGGGATTCAGAAAAGGC11.1904761904761905No Hit
GCACAATACATGTTACCGGTCAGTTGGAAAAAATACGTGAATTATGCGCTGCGTAAATACGAATTTAAACTTTCT11.1904761904761905No Hit
GTCCATACTCATCTGTTGGAAGAAGCTTAGAACCACCAACATCACTTTCCACAGGATAGACCTGTCTCTTATACA11.1904761904761905No Hit
ATTTTAAATGCTAATTCCTGTGTTGAAAATATGACTTTTCAGATATGCCGCCTCCCCAAACAAAAAATAGTGCCA11.1904761904761905No Hit
CACTAGGGGTCACCAGAGGTCACAGGGGTCAGAAGAGGTCACTAGGGGTCACCAGAGGCTGTCTCTTATACACAT11.1904761904761905No Hit
CTCCCATATCATCTCATTTTTTGCCAGTTTTGTGCTTCTATTCGGAAATGAGATGATGATCACCGCTCTCTACTT11.1904761904761905No Hit
GTTCCATGACGACCAACGTCTTCAAACTCGGACACTGCTCTTTGAACTTGAGGAGGCCGGTGGCTTTCGCGATGT11.1904761904761905No Hit
GAGGTCAATTAGACCTGCATTGCCAGCTGGCCGCGTGGCGCAATGGATAACACAGGTCCTGCGCGATCGGTCATA11.1904761904761905No Hit
TTTCAGAGCAATCGCAACAAGAAAAACGTGGAATTACAGTTGGAAAGCTCATCTGAATTCGAGAGGACTATGTCT11.1904761904761905No Hit
TTTCTACAGTTACCCCAGGCTTCTCCTTAATCTCTTTAAAGATCTCTGCTACCAGCGCTCTGCTATGAATCCCGA11.1904761904761905No Hit
GTATCTAACCTATCATCCAGCCTCGTCGTCTCCTCAAAAAGCCACTTCAAACTGATCGGTTTCTCATTGGCAGCT11.1904761904761905No Hit
CTTATTACCTTCTTAGATGTGCTGTGCCCCGGGTGAGCACACTACTGCTCCCTGGATCGCCTCAATGAGGTCCTC11.1904761904761905No Hit
CTATAGAAGTAAGCATGCAAATTACTTCTTGCCCCGTTCCCTAATGGCATAGATAGTTTCCACATACACTTTGAG11.1904761904761905No Hit
GCTCGCCTGCGGCGAAGTCCCACGGGCGCAGACCGATTTCAAAGAAACCGTCAACGCGCCCCGCAGCGCCGTAAG11.1904761904761905No Hit
GATGCGGTGTGTGTCTCGTCGGACGGTGCGTTTCGTGCTGTATGCGTGGTGTGCTTTGTGCACTGTGTATATGCT11.1904761904761905No Hit
ACTCCCAGCACAGAAACTGAAAAAGCGAGAAACATGAATGTAGTTATCTATGGTAATGTTACTCCAAACCACCAG11.1904761904761905No Hit
CTGAAACAGTGAAAAATTGCTGATTCTAAAAGTTATTAAATAATTTGTATTGAGAAGTTTAGCTATTCCGTCTTG11.1904761904761905No Hit
GAAGCAATTGTTGGTGCAACGAAAGAAGGCATAATGACAAAACAAGCGATCGTCATACAAGTAAAAGTAATTGCC11.1904761904761905No Hit
ATATCAACGTGGGGGACGCTACTGATGTTGGGGGCTCTCCTTGGATTATCAAAAATCAGATTTCTGAATATCTAG11.1904761904761905No Hit
ATTTTATATCGACTAATTTAGGTTTTAAAATACTAAGATTTCGAGTTTTTCTACTAAAAAAATCAGAAAAACTTG11.1904761904761905No Hit
GTTTATATTCGCGCTCTTCTCCTTGGCTTTTCCTCTTATTTTGCAGTGTCTTGTACTACCTGTCTCTTATACACA11.1904761904761905No Hit
CTTTTATTCTGCCTTTCACATTCCCATTAGTTCTCTTTTTTGTGTGCAACAGCCTTTCGTGTTTTTGCCACGTGT11.1904761904761905No Hit
GACCAGACACCAGTCATTGGAGTAAATACAGATCCAATTGGATCAGAAGGTCATTTATGTGTTGGTGGAAAGACT11.1904761904761905No Hit
TCATAAAATACTAAATATTTAAAAAAAAACAAAAAAAAAATTAAATACGCCCATACTCACTAACCAAATCAATTT11.1904761904761905No Hit
TCTCGTAACCGTCCGGCAGCTAAACGTGACGTTTGACAGAAAAGGACATCTAATTGGAGAGAAACACAGAAACTG11.1904761904761905No Hit
CAAAGAGACTACATCACAAAACTAATTCGAACGCTCCCCAAACATTTTACAATACCAAATCCACATAAAAATAAC11.1904761904761905No Hit
GTGTGTGGCTCAGAGTACTTAAAATATATGTAATTGTGTCTGTGTTTTGCAAGTTTTGTGACTCATTTGCTTGAA11.1904761904761905No Hit
AAATTAATATAAATCATCACTATCCAGGTGGAAACAATTGTCACAACAAGTACCTTGCCACCTGCTCCTCCATCT11.1904761904761905No Hit
ACAAACATTTTTACAAACGATTAAAGACATAAGAACGTGTAATAGTAAAAACAGTACATATCTATACTCCAAATC11.1904761904761905No Hit
CATGGGACGTTTGCAAAAACGCTGAAGAGAACGCACTGGAAGATTAGGTGATTTTTCGTTCCTCCAAAAGCAAAA11.1904761904761905No Hit
ACATATACCACTTGCTTCTTACAACATGCCATTACTGCAGAATCGTCCTCTTGCCACTCACCAACAACAGCAACA11.1904761904761905No Hit
CAAAAGAACACAAACACATTACTGATTCCGGAACCCCGGACTTCGAGATTCTTGTGGATACTCTAGACAATCTAC11.1904761904761905No Hit
GAATAAAGAGAAAGAAGAATCTAATTGCAATCTGAGACCTATAAGTGGATTAGCAGCAATATGGGATCAAAAGGA11.1904761904761905No Hit
CTCGCTAAAGAGAAGGTAACTGAGTTCTGGCAAAAGGGAAGAGAGAGAGAAAGTTTCATGAGATATTGGTGCGTC11.1904761904761905No Hit
CTCCCAGAAACATGCCTTATCCTCAATTGGATCTCCATTCGCAATCGCCCGTTCATTGCTGTCTCTTATACACAT11.1904761904761905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTG50.069.014
TTTGACG50.069.017
GTTGATT50.069.023
CAATGTT50.069.07
AAACCGA50.069.057
GTTGAGC50.069.065
GGTGAGA50.069.036
GTTTGGT50.069.011
GTTTGAC50.069.016
TTGCAAT50.069.028
AACCGAC50.069.058
AGCTTAA50.069.069
GAAACCG50.069.056
TGCAATT50.069.029
TTTCTTC50.069.043
ATTGGTG50.069.033
TGATTGC50.069.025
TGTTTGG50.069.010
GAGCTTA50.069.068
GCGAAAC50.069.054