FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2592_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2592_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173156
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGA150.002350169569.0147364
AGTGGAC252.5620344E-455.1958433
CCCGGAC200.00734233551.76104715
GCACCAA200.00735072751.74616
CTCTTGG350.001345150439.4256021
GCCCAAG459.967926E-538.34151511
ATATGTG501.8557347E-434.4974025
AGGGGCT450.004613667730.66435443
TAAGACC450.004613667730.6643544
GAGTGAC500.007719922327.5979217
ATTAAGG500.007719922327.5979213
CCATATA500.007719922327.5979211
TTAACAT658.631097E-426.536463
GTATAAT951.0446727E-525.4191381
GAACCAT700.001329862724.6416
ATACTGT1253.4771238E-622.0783376
GCGAAAG800.002881187721.57333667
AACTAAC800.002890868621.5608751
CGAAAGC850.004094355320.30431468
TCTTGGA850.004108083420.292592
Produced by FastQC (version 0.11.2)