FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2591_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2591_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318641
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCC200.00735735351.74435424
CCACGCA501.8596006E-434.49623525
ACCACGC553.2538688E-431.36021424
CCCCTAA450.004588351630.706691
ACGCACC450.00462019630.66331943
GTCTTAA605.3723424E-428.7875231
GTATGCG500.00772486427.6013213
CGCACCG500.007730784427.59698744
GAACTAA658.6408923E-426.539736
AATGCAG658.6408923E-426.539735
CAGTGCA809.547662E-525.8721779
ATAATAC1001.5584807E-524.1511553
TAAGCCA1301.8406172E-723.8820134
TACACAG1052.273249E-523.0010995
CACGCAT800.002896718721.56014626
TTCTGCG800.002896718721.5601467
CCGTCCG850.004116355420.291948
ATACAGC850.004116355420.291931
GTATAGA1206.304947E-520.1512661
ATTCTGC1206.3662155E-520.1259616
Produced by FastQC (version 0.11.2)