FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2586_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2586_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176136
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCGT150.002353187968.99295
TAATGTC350.00134539539.4245154
GTACCGT450.004614499430.6635156
ATATGGC450.004614499430.66351550
GTCATAT500.007699928727.6128391
TATAGAC500.00772131327.597162
GTATAAT809.486755E-525.887041
CTATTGA700.001325767824.6543221
AGGAGCC700.001330166224.64032228
TATAATG950.007813456518.1560272
CTATTCT1150.001105919717.998154
CAATTTG1250.001933161916.55829833
ATTATAT1350.003230715615.3317563
TATTCTC1605.728267E-415.0921975
TCCTATC2506.008855E-613.8064269
CTCCTAT2506.008855E-613.8064268
TAGGGGT2751.0637177E-613.798584
CACTAGG2355.1547933E-513.2189141
CTATCTC1850.001709315813.06012669
ACGGACT5400.012.78372369
Produced by FastQC (version 0.11.2)