FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2576_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2576_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179657
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGTC200.0073508651.74666217
GGTAACC200.0073508651.7466628
ATAGGCA459.985769E-538.3308646
TAGAGCA400.002591352234.4977764
CATAGGC553.2474136E-431.36161245
ATACTCC605.406281E-428.7481446
CCTAATA500.007720276227.5982192
GGCATAG700.001329973724.64126843
GCATAGG700.001329973724.64126844
GTTATAA953.0415648E-421.8002031
TAGGCAT800.002891105821.5611141
CAATAAG1004.3009027E-420.7101921
GTATTGA850.00409518420.304111
CTATTAT1206.324767E-520.1349091
AGGCATA900.00571813419.1654342
GAATATA950.00778727118.1668361
GTATATT1150.001105753417.9988383
GTATTCT1300.002502378815.9309171
AGGAGAG1350.003230228815.3323445
TAAAACT1350.003230228815.3323444
Produced by FastQC (version 0.11.2)