FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2572_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2572_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108910
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGTA150.002350120868.997742
TAGGATG253.0762512E-668.99775
CTAGCGC150.002350120868.99773
GTCGAAC200.00734222151.74827642
TAAACCG200.00734222151.7482765
CCAAGAT200.00734222151.74827661
GGTTGTT400.00258649334.4988541
TCATACT450.00460527830.6656484
GTCTCAT500.00768870127.611761
GAGAGCG500.007705966527.59908163
AGAGCGA500.007705966527.59908164
GTATTTT900.005687814719.1748331
AATGTGA900.00570294419.1660296
CAAAGCC900.00570294419.16602945
AGCGAAA1350.003208807715.33986666
CTCTATT2051.4195182E-515.14583621
GAATGAA1400.004087393614.7920141
CCTCTAT1901.2651915E-414.52583320
AGTGACC1709.024933E-414.2054113
GTCATTT1500.006480389713.79954059
Produced by FastQC (version 0.11.2)