FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2567_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2567_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96726
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCC200.007328551351.76823468
AGCGCAC200.007328551351.76823410
TATCCTT200.007328551351.76823410
GAGCGCA404.957633E-543.1401989
AGAGCAG350.001342798739.422075
CAAGCCA400.002586732934.4943124
AGTATCC450.00459406430.6774758
TTGGAAC605.391224E-428.745263
TATGCCG850.004097298720.29077143
TTAACTG900.00570274919.1635075
ACGATTT1301.16373434E-418.5834713
CGATTTC1250.001925470316.5572714
TGGTGAC1500.006456915313.80486312
GGTGACC1550.00802151313.35954513
CTAAGCC1550.00804902513.3526373
CTGGTGA1600.00989289912.94205911
TACAGTA1600.00992676512.9353675
GTGACCC1600.00992676512.93536714
ACGGACT3501.1926495E-611.8327469
CGGACTC2954.2496575E-410.52913369
Produced by FastQC (version 0.11.2)