FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2562_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2562_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370576
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT601.3363691E-534.514041
TTATACG605.415138E-428.7500554
GTCCACC500.007733285427.59632938
GTATTAT1651.1459633E-1025.101121
CAGCGTC700.001331079824.646232
TAGTATA700.001332127324.6429044
ACAGTGC851.4407058E-424.3529878
TATACTG1554.067624E-822.2581085
TATGGGT1103.2591633E-521.954594
TATTAGG800.002893464921.5654532
ATTAGGG800.002895732421.5625423
TATGACC1808.520146E-921.0862259
ATAGGGG1004.3271153E-420.7000393
GTATAGA850.004105313720.3023761
GCGTCAG850.004105313720.3023761
AGCGTCA850.004114953320.2941573
GGTTTAG1055.9959694E-419.722311
GTGTAAT1055.9959694E-419.722311
ACAAAGG1056.012351E-419.7143253
CCTACAG900.005731643619.16411837
Produced by FastQC (version 0.11.2)