FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2559_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2559_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144110
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTCC252.5610975E-455.193619
ATGCAAT350.001344666539.4240076
GGACACC400.002590306334.496016
TGGAGAG1151.6822214E-623.9972255
CTTTAGA800.002871793221.5824721
CTAGAGA800.002889215221.5600054
AGGACAC800.002889215221.5600055
AAGCCTA800.002889215221.5600055
CATCTCG1808.36917E-921.09553569
GCATCTC2501.8189894E-1220.7119869
TTTTAAC850.00408104120.3129162
TAGAATT900.005714440319.1644524
GAACACA900.005714440319.1644526
TGAGCAT5900.017.55252569
GGGGTCA2001.1294345E-515.5232036
AGCATCT3503.6379788E-1014.794270568
CTGAGCA7300.014.18628768
CTCCTGA12000.014.09565369
GAGCATC4253.274181E-1113.80798569
CCTGAGC7650.013.53724267
Produced by FastQC (version 0.11.2)