FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2556_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2556_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119406
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG252.5578504E-455.25
ACAACCC200.007342980251.7500043
GACCATC200.007342980251.75000411
ACGACCA200.007342980251.75000422
GGGACAC400.002587066334.557
GTCCATC450.004606290330.66666649
GTTATAG450.004606290330.6666661
AATGTAA450.004606290330.66666650
TTTAGGG605.393519E-428.7500022
TAATACT500.007707645727.64
CGTCTGT500.007707645727.669
TCTAGGA500.007707645727.62
ACCTCTC500.007707645727.617
CTATTAT500.007707645727.61
ACATACA500.007707645727.64
CTGGAGC658.611538E-426.53846226
TTTACAG800.00288440721.5625023
ATTATAT800.00288440721.5625023
CCCTGTG1258.657715E-519.3200022
CCCCTAG2851.6370905E-1118.1578961
Produced by FastQC (version 0.11.2)