FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2555_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2555_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144679
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT150.00235137669.000011
GTACCGT200.00734610551.7500046
CGTTGCT200.00734610551.7500042
ACCCTTG350.001343919239.428577
GTATTAG400.002588869834.51
TAATACA450.004609482430.6666664
TCGATAG450.004609482430.6666663
TGTTCGG450.004609482430.6666668
CACATAC500.00771295227.61
TTACTAT500.00771295227.610
ACCAGTC500.00771295227.635
TCTACTC809.505302E-525.8750023
TCTATAC851.4348047E-424.352943
CTTGATC750.001984660523.03
CTACTCA800.002887329321.5625024
GGGGTAC1258.673897E-519.326
TTCAGGT900.00571072719.1666664
GACCCCA900.00571072719.16666619
CTAGTGA1108.189292E-418.818184
ATCATAT1108.189292E-418.818181
Produced by FastQC (version 0.11.2)