FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2554_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2554_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128886
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCGT150.002347645369.0232958
GGCGCCG252.5592535E-455.19720523
CATGTAC200.007345764451.747383
ATTACGT350.001341143539.4418831
TGCGGAC350.001343706739.4265812
TGTTATA350.001343706739.426582
AGTACCG350.001343706739.426585
ATAGAGT450.004608772730.66511551
ACAGGTG450.004608772730.66511547
ACAGTAC605.3974614E-428.7485473
ACAATGT500.0077117827.59860224
TAGATGA700.001327825124.6416114
CTATTGA900.005696278519.1731381
GTCTAGA1300.002499804115.92845251
TAAAACA1350.003223306315.3325584
TCCTGAG3001.8530773E-713.80465969
GCATCTC1500.00647296613.80465969
TATAAAA1600.00994273612.9368452
GTGACCC2150.005275857611.23198927
GACCTCT3401.13474725E-511.16129
Produced by FastQC (version 0.11.2)