FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2547_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2547_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146863
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGG200.007335140451.7699362
TAGTAGC200.00734503251.75239
CGTGGGA200.00735493351.7346829
CTTGGGG350.001343690439.4303253
ATTACCA400.002588431134.50153436
AGATCGC450.004608702430.6680310
CCCCTCA500.0076988327.6106321
TACTCCA500.0076988327.6106325
CACACTC500.00772448227.59183131
TATACTC953.040101E-421.7978655
GTTAAGG800.002881082521.5708051
TGGCTCC950.00781613718.15251748
TATTGAA1200.001468052717.2507652
ACGCAAT1500.00650591713.79591515
ACATACG1600.0099675212.9336711
TTAGGCT5602.1827873E-1111.7098674
TGAGCAT2450.001075815311.27732969
CCTGAGC2450.001075815311.27732967
AGGCTTA5057.208655E-910.9349036
CCTCTAT3501.5672942E-510.8433399
Produced by FastQC (version 0.11.2)