FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2544_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2544_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140722
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGT200.007348533751.74493440
TACAGAC306.2845065E-446.011852
TATACAG400.002589873334.4966245
ATTAGTA450.0046032530.6745662
GAATAGC500.007715931627.59736
AACACCA500.007715931627.59735
ATATAGA850.0041048520.2921316
ATAACAC950.007789629518.1625733
GTTCTCT1150.001096494718.0174471
TCCAGAA1750.001123998213.8035542
CCTAGTG1550.00805045613.3582793
GACTCCT3952.248271E-913.09998469
CCTGAGC1850.001713595813.05277769
CCCTAGT1600.00992844812.9408332
ACCTCGC1600.009951781512.936233517
CACCTCG1600.009951781512.936233516
AGACTCC4601.6771082E-911.99882568
TAGAGAG2357.65802E-411.7435315
ACTCCTG3308.162975E-611.49887569
TCCTGAG2200.006267840510.97619868
Produced by FastQC (version 0.11.2)