FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2542_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2542_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135269
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTT150.002351744368.9948247
TGGACGC200.007347257351.74611745
CTGCCAC200.007347257351.74611756
GGACGCT200.007347257351.74611746
CCCCACT200.007347257351.7461177
CTGACCG350.001344104639.4256179
TATGACC459.973294E-538.33045659
GGGTTCA501.8535879E-434.4974149
AGTCAGA450.00461012530.66436627
CCCAATA500.007700114527.6081372
TAGGACA1052.2564298E-523.006784
CTAGGAC1004.2990543E-420.7061023
CTCTAGG1055.943775E-419.7346941
CTATTCT1055.9735036E-419.7200974
AGGACAC1258.648944E-519.3256955
TCTAGGA1200.001464781717.2550852
TCTCTAG1200.001468417717.24870535
GGACACA1452.6941224E-416.6600826
TGAGCAT1452.6941224E-416.66008269
TCCTGAG2557.3561587E-613.53340169
Produced by FastQC (version 0.11.2)