FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2525_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2525_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157481
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGGG200.007348110451.748578
GTGTGGG400.002581853734.5209731
TTAATCC400.00258991534.4990463
TATATGG553.2449004E-431.362772
GATTAAA500.00769213427.6167771
TGTCACC500.007716033627.5992378
TGGGGGG500.007716033627.5992375
CTCGAAG500.007716033627.59923712
TAATAGA800.002888917221.5619034
TCACAAT800.002888917221.56190313
AAGGATA850.00410531820.2935585
TTAGAGA1055.9925026E-419.713744
AGGCATA900.00571383919.16613842
TCTGTTG950.007806484618.15739459
ATTCTCA1600.00995405312.9371426
CAGGCAG3852.2129825E-812.54909369
GACAGGC4752.7721399E-911.62442369
CGAGACA6950.011.42057868
ACAGGCA4258.7522494E-811.36800268
AGGCAGA3105.4689277E-511.13225969
Produced by FastQC (version 0.11.2)