FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2523_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2523_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148272
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCAC150.002346342269.0381368
TCGGTCC306.297385E-445.9943828
CTGGGTG350.001344834939.4237569
GTGTAAT400.002577792434.530721
TTATACT905.114998E-934.5074234
TGTGGAC400.002590629534.49578510
CCATAGT553.239373E-431.3703844
ACTATCT553.2457925E-431.3598048
TCAATAC605.3929375E-428.7561863
TCCATAG500.007705461727.6059383
GTGTATA658.627573E-426.535229
TATATTC700.001326711424.6481573
TTTATAC700.001326711424.6481573
TATAACA700.001326711424.6481574
TACAACT700.001326711424.6481572
TATACTG851.4329435E-424.358185
GAATATA1202.4151486E-623.0204811
TATACTT750.00198240823.0049485
CTATATG750.001986303622.99718945
AACTCAC800.00288971421.5598647
Produced by FastQC (version 0.11.2)