FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2521_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2521_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161108
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAT200.007350750751.74441531
TACAGAG450.004613460530.6633577
TACTAGA703.8335485E-529.5682372
GTAATAC605.4052065E-428.7468973
ATCCTTG500.007707891527.60558753
TGTATAC500.007719583327.597023
TAGTTAT500.007719583327.597026
TATACAG500.007719583327.597025
TAATACA851.436847E-424.3503134
TTATACT1001.5541478E-524.1473924
TAATACT800.00289055121.5601734
CCTCAAT800.00289055121.56017343
TGTAATG800.00289055121.56017312
CTTATTC850.00410763620.2919273
ATAATAC900.00571705719.1645983
TCTTATT1350.003229227815.3316772
TTATTCT1550.00807662713.3533964
GGCAGAA3003.919419E-511.50232969
ACCTCTG3359.673953E-611.3271357
CTCTGGT3451.3408509E-510.9988129
Produced by FastQC (version 0.11.2)