FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2516_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2516_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62043
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGCA200.007326417651.7528
CGACTGA200.007326417651.7520
CCGTAAT350.001337987639.4285741
TTAGATT450.004589387730.66666640
AAGAATG450.004589387730.6666667
ACAGGCA1951.5601538E-414.15384769
GACAGGC2001.9343286E-413.868
CAGGCAG1600.00986353912.937569
TTTGACC2050.003659024811.78048815
AGACAGG2357.546083E-411.7446867
GCCCACG5451.5825208E-1011.39449669
TTGACCT2200.00619656410.97727316
CCCACGA4451.5897058E-710.85393269
AGCCCAC6008.5128704E-1010.3568
GAGCCCA6352.2737368E-99.77952867
GAGACAG2850.00376574079.68421166
CGAGACA2850.00376574079.68421165
CGAGCCC6951.0746589E-88.93525266
ACGAGAC3150.0085814368.76190564
CCGAGCC7302.4869223E-88.50684965
Produced by FastQC (version 0.11.2)