FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2514_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2514_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94371
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATT200.007327303351.7694857
GGCGTGC200.007342688351.7420520
GCGGGGG200.007342688351.7420556
TGATATA459.954972E-538.3274462
GATATAT400.00258627334.4946983
GTTATGT450.00460489830.66195522
AGTCAAC500.00768542627.61039211
GATAAAG500.007705352727.5957581
ATGATAT658.605791E-426.5343861
ACAATGC809.446698E-525.8847438
ACTGTAC750.00197523723.008668
CAATGCG750.00197523723.008669
TATGACA800.002882535621.5591874
GACAATG850.00408373620.3017587
CAGGCAG2603.1754098E-815.92907269
CAGAGGT1657.192884E-414.63411417
ACAGGCA3554.4201443E-1014.58295369
CCAGAGG1951.5759504E-414.1516716
ACCAGAG2102.9537763E-413.14083915
CACCAGA2153.60365E-412.835237514
Produced by FastQC (version 0.11.2)