FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2512_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2512_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75017
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGG150.002347130368.9986652
TAGACAA200.00733294751.74925
ATATGGT350.001339912539.4278074
TAGGACA350.001339912539.4278074
GTGTTAA459.888602E-538.3581661
CCTGTTC500.007690389627.5994648
ACAGGCA3209.094947E-1116.1823569
GACAGGC4002.5975169E-912.9458869
AGACAGG4803.1213858E-911.5074569
CGAGACA6301.9990694E-99.86352769
GAGACAG5907.824565E-89.35575168
ACGAGAC7252.2555469E-88.56535268
CACGAGA7757.723429E-98.45790167
CCACGAG8554.4343324E-87.666518766
CCCACGA9453.2528988E-87.30631769
GCCCACG10351.7424463E-76.666537368
AGCCCAC11257.861563E-76.133214567
GAGCCCA12102.881614E-65.70236966
CGAGCCC12354.137124E-65.58693765
CCGAGCC13301.5160196E-55.187869564
Produced by FastQC (version 0.11.2)