FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2511_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2511_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120031
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTAA150.002350509668.9991713
TCATAGG200.0073434251.749382
CTGTACT350.001343083939.4280934
ATCTTAG350.001343083939.42809347
TGTACTT450.00460665430.6662965
GAAGTAT658.6124E-426.5381416
TGAAATG1403.7181962E-722.1783035
GCAGAAA2650.020.82993769
ATATTAT900.00567812319.182421
CAACTTT900.005705567519.1664356
CCCCTAG1552.203312E-517.8210871
CCTAGTG1653.7959828E-516.727073
GGCAGAA3006.566552E-1016.09980668
CCCTAGT1605.7054666E-415.0935692
AGGCAGA3253.277637E-813.79983467
CAGGCAG3508.650932E-812.81413166
TTGAAAT2255.299499E-412.2665194
ACAGGCA4004.9067785E-711.21236465
GACAGGC4652.51257E-811.12889869
TAGGCTT3551.827111E-510.6900125
Produced by FastQC (version 0.11.2)