FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2501_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2501_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77225
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA150.0023416269.041481
GATAGGG200.007315778651.7811051
TATAGGA200.007334560651.747574
TCCCATA200.007334560651.7475751
TATAAAC350.001340339139.426723
ATGTCTG450.00459736130.66522861
CTAGATC800.002865244221.5754669
CCGTACT2850.020.59131869
GGGTCAT900.005688661219.1657687
TTGGGGT900.005688661219.1657684
ACCGTAC3300.018.82949468
GACCGTA3650.017.969767
ACTAGAT1250.001910899516.56995468
AGACCGT4150.015.80467666
TACTAGA1350.003193761715.3425567
GAGACCG4801.8189894E-1213.65560865
GTACTAG1600.0098530612.94527666
CGAGACC5207.094059E-1111.94174864
ACGAGAC6051.0059011E-910.26398163
CACGAGA6503.4815457E-99.55339762
Produced by FastQC (version 0.11.2)