FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2499_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2499_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223153
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTTC400.002592532734.49820735
TTATAGT652.3140274E-531.84454
GCATAGG1001.4395482E-831.04838644
CCTGTAC450.00461596830.6650733
CCCTGGA450.00461596830.6650732
CATAGGC802.7496299E-630.18593245
TGGGCGA605.410032E-428.74850510
TATACAG756.143798E-527.5985645
GCACGCC906.876304E-626.8319422
CTAGGTG658.637773E-426.5370834
TCGCACG809.531385E-525.87365520
CGCACGC809.531385E-525.87365521
TCCTGTA700.00133088624.6415772
AGTGCCT700.00133088624.6415778
GGCATAG1151.6882077E-623.99875343
CAATACT953.0559962E-421.788344
ATGGGTG800.002893067221.561385
CATTATA800.002893067221.561381
CCTATCC800.002893067221.561383
CAATGCG1154.583157E-520.9989099
Produced by FastQC (version 0.11.2)