FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2498_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2498_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211841
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAC400.002591575734.56
TATACTA500.00772091427.65
AACACCG809.5257754E-525.8755
GAGTAGA700.001330259624.6428591
TAATATA800.002891714721.56252
GTGTACA800.002891714721.56259
GTATAAA800.002891714721.56251
CTCTAAT850.004109272320.2941171
TTAAACT900.005719310619.1666664
GTCGAAT1150.001106163618.01
ACAGTGA1250.001933577916.568
TACTAGA3551.8189894E-1216.52112869
TACACTT1503.5081862E-416.15
ACACTTA1350.003231392515.3333336
ACTCATC1350.003231392515.3333338
ATAAAGT1657.2369823E-414.6363633
AACAGTG1450.00519631814.2758637
ATTACTG1709.0724305E-414.2058823
ATAGCTG1550.00808188413.35483950
GTACTAG4406.002665E-1113.32954668
Produced by FastQC (version 0.11.2)