FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2497_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2497_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182688
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGGC200.007350565451.74753655
CACAAGC200.007350565451.7475367
ACATTAA350.001339736339.4591031
TTATACG350.001345160939.4266974
GTCTACG400.002587772234.50782
GTACAAC400.002591250734.4983563
CCGATTA400.002591250734.4983569
CCATAAC450.00459518230.690411
TTGTTAC450.00459518230.690411
TTAAACC605.4060953E-428.7486324
AACAGCG500.00771997127.5986867
TACGGAG658.617726E-426.5444642
GTAAAAC851.437287E-424.3517823
ATACGGA750.001977721523.0178071
AGTATCT750.001987198622.9989058
TTATAGA800.002877247821.5791951
TATATAC1154.5674977E-521.0047512
TAAAACT1154.577152E-520.9994
CTATATA850.004088762720.3098321
GTTAAGG850.004088762720.3098321
Produced by FastQC (version 0.11.2)