FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2496_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2496_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185743
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCC200.00735005351.7487874
ACCCAGC200.00735005351.74878750
GCTTGTC400.002591053534.4991921
CAACTAG553.2470498E-431.3629024
TAATACT450.00461334930.6659494
ATAGCAT450.00461334930.6659493
CTAATAC450.00461334930.6659493
TTAGGCA658.630848E-426.5378440
TAGGCAT1101.1443299E-625.09032241
GGCATAG1052.2678918E-522.99946243
AGGCATA1253.4778077E-622.07948542
GCATAGG1103.2485244E-521.95403144
CATAGGC953.0527328E-421.78896345
CTATAAA850.0040951520.3045791
GTAATAA900.005699707719.1765421
GATTGAG900.005699707719.1765421
ACAAGGA900.005717485719.16621838
TCTAGAC1108.202561E-418.8177413
CCCTGTG2359.333235E-917.616612
CCTGTGA2351.7937782E-716.1485583
Produced by FastQC (version 0.11.2)