FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2495_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2495_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170853
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTTA150.002353096668.992684
CCCCGGC200.007342946751.75966362
GCCCATG200.00735145351.74451418
GTCTCCC200.00735145351.74451441
TTGCCCA200.00735145351.74451416
GTATTAT306.2814355E-446.022061
CCCTACT553.236404E-431.378681
CTCCGCA450.004614189730.66341613
GTGTTAT500.00769875727.6132371
CGCATTG500.00772079727.59707616
CCTACTG500.00772079727.5970762
TGCTGAC500.00772079727.59707645
TATACTT500.00772079727.5970765
GGATTAT658.6026907E-426.5511881
TAAGCCA1251.2527016E-724.83736646
GTTCTAA700.001325495724.6546751
ATGAAGT700.001330028624.64024513
TCGACCT700.001330028624.6402455
GTGCCCT750.001983968323.00429557
TCTAACC750.001987350222.9975623
Produced by FastQC (version 0.11.2)