FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2491_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2491_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134326
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGCC150.00235141168.99702548
CGGCCCC200.00734622151.74776514
AACTGCG200.00734622151.7477657
CCTTCAC306.292804E-445.9980163
GTATTAA350.001341401339.4415551
CGCCCAC350.001343860839.4268714
GTATACA652.3012055E-531.8566381
ATGTAGA450.00460089830.6767641
TAGGCAT450.00460929330.66534241
CCCTTCA500.00771264427.5988082
TATAGAG500.00771264427.5988085
TATACAA658.619345E-426.5373152
ATAATAT900.005710066319.165843
AATAATA1200.001468031317.2492562
ATACAAA1250.0019291716.5592863
TGACCTT1350.003224097215.33267128
ACTAGAT2551.1087321E-412.17594569
ACGAGAC8100.011.07359769
CCGTACT4157.9220445E-710.80676369
GGTGACC3301.0329983E-410.45409516
Produced by FastQC (version 0.11.2)