FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2487_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2487_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63675
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTTT150.002345736668.9968664
AAGCGTC200.007305866651.78832666
GTCATAA200.007328628651.7476461
GGTCAAC350.001338556239.4267731
TTTACAC400.002578604334.498433
TTTCCCC500.00768275727.59874342
TGATGAA500.00768275727.59874332
CTAAGGC1402.0323554E-417.26277569
ACTAAGG1452.6569807E-416.66750768
GACTAAG1850.001685008413.06372367
CACGAGA3701.7174716E-712.13059969
CCTCTAT2100.004384615511.499475531
CCACGAG3954.0134364E-711.36283968
AGACTAA2150.00519531511.24087766
AAGCCTA3207.416903E-510.78075917
AGCCTAA3207.416903E-510.78075918
CGAGACT2600.001747906410.62324669
TAAGCCT3055.7417696E-410.17986410
CCTAAGC3106.668432E-410.0156732
CCCACGA4552.4772125E-69.86444367
Produced by FastQC (version 0.11.2)