FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2484_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2484_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174387
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGGGT150.002353157868.992834
CCGGTCG200.007343306751.7594689
TGCGTTA200.007351642551.74462514
TAGGGTG306.299886E-445.995225
CCTAATA350.001345365339.4244772
CTTAGAG350.001345365339.4244773
TAATACT557.363662E-637.6324544
AGTGGGG553.2423114E-431.3693738
ATAGGCA553.247773E-431.36037852
AGTGTTG553.247773E-431.3603785
CTAATAC450.00461439930.6634833
CAGCACT500.00772114427.5971324
CTAATAG500.00772114427.5971323
TATCCAC700.001330121524.6402975
CCCTGTG1301.8255196E-723.88898312
CCTGTGA1451.9861545E-823.79745713
AAGTGGG902.113693E-423.0042087
ATATGTG850.00410887520.292015
CTGTGAC1559.954747E-720.03017614
AGAAGTG1055.999129E-419.7122365
Produced by FastQC (version 0.11.2)