FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2480_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2480_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91264
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCA150.002348660768.9994652
TAACACC200.00733768851.749594
CATATCG350.001341365239.4282626
CTACCTC350.001341365239.4282634
CTTACAT350.001341365239.428263
ATGAGCA601.3291596E-534.499739
CCTCGCA400.00258398134.4997253
TTATACT501.8481442E-434.4997254
ATTATAC400.00258398134.4997253
TATACTC501.8481442E-434.4997255
TACTTTC450.004600832230.6664227
GTATAAT500.00767798527.6149121
ATCCAAT750.001972861623.0124281
TGAGCAA902.1056949E-422.99981710
GGAAAAT900.00569516719.16651329
TAAGGCG1602.902705E-517.24986369
AAGGCGA1300.002498059815.9229569
CTAAGGC2102.948475E-413.14275368
ACTAAGG2409.076148E-411.49990967
TTAGGCT2500.001277200111.0399134
Produced by FastQC (version 0.11.2)