FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2478_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2478_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48798
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACT200.007317064351.751
AAAGTTC350.001335172339.42857416
GTGGAAA500.00766367327.61
TCGTACA500.00766367327.621
CTAAGGC1350.003183950715.33333269
ACTAAGG1450.005120714.27586268
GACTAAG1951.5493298E-414.15384769
AGACTAA2408.9431804E-411.568
GAGACTA3055.6877866E-410.18032869
CGAGACT3250.00101922669.55384668
ACGAGAC3350.0013447319.26865667
CACGAGA3600.00258879488.62566
CCACGAG3600.00258879488.62565
CCCACGA3800.00422112748.17105264
GCCCACG4050.0074826687.66666763
Produced by FastQC (version 0.11.2)