FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2477_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2477_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77352
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGACC150.002347229569.041
TTACGAA150.002347229569.030
ACGGCTT200.007333251651.75000453
GGTTAGG200.007333251651.7500041
CCTAGAG450.0045963630.6666663
TCAAACC750.00197636323.03
TAAGGCG1808.272764E-921.08333469
GGCGAAT850.004086094420.29411768
CTAAGGC2104.8590664E-818.0714368
TAGAGAG1351.5530297E-417.888895
AAGGCGA1704.8655194E-516.23529269
ACTAAGG2605.5227065E-714.59615467
GACTAAG3002.766934E-612.6566
AGACTAA3802.479519E-711.80263169
GAGACTA4251.0536169E-610.55294168
CGAGACT4703.823625E-69.54255367
TAAGCCT4108.786183E-59.2560984
CCTAAGC5008.393559E-68.972
GCCTAAG5059.520434E-68.8811881
ACGAGAC5101.0783793E-58.79411866
Produced by FastQC (version 0.11.2)