FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2476_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2476_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126064
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCAC252.5583996E-455.1999973
TTACTCA200.00734392551.754
TTATCCC200.00734392551.754
ACATGAT200.00734392551.7553
ATATCCC400.002587611634.51
ATATTAA652.3052775E-531.8461531
ATAATAC450.004607254630.6666663
TCACCTT703.8241837E-529.5714265
TGCCTTA605.395207E-428.7555
TACATCT500.007709250327.59999835
ACTAAGC500.007709250327.59999850
GTGTCAA700.001327273824.6428571
ATGCCTT750.001983251223.054
TAAGCCA1352.6204907E-723.034
GCCTCTA1253.4624263E-622.0846
TGCCTCT1253.4624263E-622.0845
TATAATA800.002885290221.56252
TCTGAAC1206.3295636E-520.1258
TTAATGA1258.662605E-519.324
TTCGAAC900.00570673419.16666649
Produced by FastQC (version 0.11.2)