FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_qg2475_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_qg2475_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81442
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGAT150.002347672869.023
CGAGCCA150.002347672869.058
TCGATCT200.007334627751.7550
ATGGTAA200.007334627751.7568
TAAGCCA450.004597761730.66666646
CTAGGAC800.00287660321.56253
CAAAGCC800.00287660321.562551
GGACACA850.00408790720.2941176
CTCTAGG900.00568972619.1666661
TAAGGCG1351.5542979E-417.8888968
AAGGCGA1402.0527936E-417.24999869
CTAAGGC1605.6769745E-415.0937567
ACTAAGG1851.0029084E-414.9189269
GACTAAG2052.3992723E-413.46341468
AGACTAA2601.3160212E-411.94230767
CGAGACT3308.0365335E-611.569
GAGACTA2752.2176502E-411.2909166
TAAGCCT4703.4328878E-710.2765954
AAGCCTA4753.9698716E-710.1684215
ACGAGAC4107.936478E-610.0975668
Produced by FastQC (version 0.11.2)