FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_nic894_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_nic894_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9998802
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAG189300.016.46503869
ACGTAGA152200.015.67072369
CGTAGAG115300.015.38719569
GTATTAG19500.014.3361521
GTATTAA20050.014.11502551
AGACGTA241650.014.11223469
GTATATA28200.014.0744331
TATACAG23600.014.0332735
TTATACT43000.013.4784694
GTATAAG20400.012.8577691
GAGACGT313250.012.81487269
GTATAAA42650.012.7855731
GTATTAT34300.012.7788511
GTATAAT40850.012.7575431
GTCTAAG10950.012.6074731
GTAGAGG98350.012.56418469
CTATACT17950.012.4924784
TCTATAC22000.012.3880213
ATTATAC42950.012.2892613
ATACTGC20300.012.0659646
Produced by FastQC (version 0.11.2)