FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_nic535_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_nic535_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGATGGATGTAATTGATGCTGAAAAGCTGAAGGAAGAGTTGAACATTGGACAGTTAGAAGAGCCTGAAGAA17.6923076923076925No Hit
TCTGAGAAGAGATCGCTGAGCAAAGCTCCTAATTAGAGGTGCTGGCCGGCACGAGCCCAATATTCTCGAAAACGG17.6923076923076925No Hit
TCTTTAGAGTTCTTATTTTTAGCTTTCACTCATCTTCTAATATCAACATCATGAATACTTCTATTACTGTCTCTC17.6923076923076925No Hit
CTCGTAGTATGACGAATCCCGGATCGAAGAGGAGCGACATGACTCCTGTAAAAACTGTGTTTTCAAAAGATAATA17.6923076923076925No Hit
GTTTAATTCTCTCATCTGCATCAGGACTGTCTCTTGGAAAGGAAGGACAAATGGTTCATCTTGCTTGTTGTATAC17.6923076923076925No Hit
AACTAATGGCGCGTGAAATAATATATTTGAAAACTTCGGGAGCCAGAGGAGCTGGATCTTTTAATAATAAATTTA17.6923076923076925No Hit
AATTTTAAGTTTGCAGATTCTGAGATCAATAATCCAAACATTCCCAAATCAGCGACCTTTTTCTTGCCCTAGTAC17.6923076923076925No Hit
CTCATATTCCAATTCGAAGGGTATTCCCCCAAAAAACATGAGTCTAGACCCGAAAACTTGATGTTTTCCCACGAT17.6923076923076925No Hit
ATGCCTTCACTGCTATCAATTTCCGGCTTACGTTGCGTTGTCATGGAATCATCTTTAAGCGACGTCGTTTAACAG17.6923076923076925No Hit
TGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATATTGAAA17.6923076923076925No Hit
AATACTCTCCATAAGTCTTCTTAATTTTTGGCGAATTCGGCCAATTATCCACCTGGCAATAGGTTTTACGCATCA17.6923076923076925No Hit
TCCTAGAACGGGTGGAGTGAAAAGAGACGGGAAACCGTCCGGTTGTGAATACTCCGTTAATACTGTCGGAAGTTT17.6923076923076925No Hit
CCCTGAAAAAACACGCTTTTCGGGTTTTTGAGAAATTTTTGAACTAACGTTCAGTTACTGTCTCTTATACACATC17.6923076923076925No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers