FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_nic1192_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_nic1192_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14445683
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGAAT173100.024.52532469
TAGAGGA338250.021.46914169
ACGTAGA844400.018.9701269
CGTAGAG611250.018.88791369
GTAGAGG447250.018.729569
AGAGGAA300350.018.52439969
GACGTAG1130550.017.80774569
TATACAG23350.016.5477665
AGACGTA1481750.016.17722569
TACACCG20250.016.0144145
TATACCG19100.015.353025
GTATTAT35000.014.692881
TACACAG17950.014.4146815
GTATTAG18250.014.3727331
GAGACGT1869600.013.82922569
GTATAAA49500.013.805391
TTATACT44900.013.5230474
TATTAGG22150.013.0839642
ATAATAC39050.012.6336623
CTATACT17000.012.3791024
Produced by FastQC (version 0.11.2)