FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_ju3170_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_ju3170_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6138152
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT19750.015.7217374
GTATATA19100.015.5413361
TACTATA13050.014.80528452
GTATTAT26750.014.7097181
CTATACT11750.014.3874334
TATACTG21650.014.1826445
TTATACT35050.014.1742334
GTATAGA17700.014.0404971
GTATATG17300.013.7665851
GTATAAT32750.013.4903091
ATAATAC25150.013.3063473
TATTATA33000.013.2778892
GTCTAAG7650.013.0845811
GACGTAG59000.012.98661369
TATAATA33800.012.9636192
GTATTAA16250.012.956861
GTATTAC14200.012.8828151
GTAATAC13800.012.5001733
ATTATAC33250.012.347543
GTATAAG12200.012.1655541
Produced by FastQC (version 0.11.2)